Rapid Tagging and Integration of Genes in Giardia intestinalis
نویسندگان
چکیده
منابع مشابه
Rapid tagging and integration of genes in Giardia intestinalis.
We developed a series of plasmids that allow C-terminal tagging of any gene in its endogenous locus in Giardia intestinalis, with different epitope tags (triple hemagglutinin [3HA] and triple Myc [3Myc]) and selection markers (puromycin, neomycin, and a newly developed marker, blasticidin). Using these vectors, cyclin B and aurora kinase were tagged, expressed, and localized.
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The reproductive mechanism of Giardia intestinalis, considered one of the earliest divergent eukaryotes, has not been fully defined yet. Some evidence supports the hypothesis that Giardia is an exclusively asexual organism with a clonal population structure. However, the high genetic variability, the variation in ploidy during its life cycle, the low heterozygosity and the existence of genes in...
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ژورنال
عنوان ژورنال: Eukaryotic Cell
سال: 2011
ISSN: 1535-9778,1535-9786
DOI: 10.1128/ec.00190-10